Journal: EFSA Journal

Article Title: Dietary reference values for chloride

doi: 10.2903/j.efsa.2019.5779

Figure Lengend Snippet:

Article Snippet: Wack et al. ( ) , 30 (140) , USA , 0–60 days , 12.93 ± 3.49 , 459 ± 124 , , Samples collected by hand expression or breast pump from a single breast. Chloride determined by a potentiometric method using a Buchler Digital Chloridometer.

Techniques: Concentration Assay, Expressing, Colorimetric Assay, Titration

Journal: Journal of molecular endocrinology

Article Title: clc-2c is regulated by salinity, prolactin and extracellular osmolality in tilapia gill

doi: 10.1530/JME-17-0144

Figure Lengend Snippet: Effects of hypophysectomy (Hx) and ovine Prl (oPrl) on plasma osmolality (A) and Cl− (B) and branchial gene expression of ncc2 (C), clc-2c (D) and clc-3 (E). Mean ± SEM (n = 8–9). Gene expression is presented as a fold-change from the saline-injected sham group (open bars). While held in brackish water (12‰), fish received three intraperitoneal injections of oPrl (5 μg/g body weight) (solid bars) over 5 days (see ‘Materials and methods’). Sham-operated (open bars) and Hx fish (shaded bars) received saline injections. Means not sharing the same letter are significantly different (one-way ANOVA, Tukey’s HSD test, P < 0.05).

Article Snippet: Plasma was separated by centrifugation for measurement of plasma osmolality using a vapor pressure osmometer (Wescor 5100C, Logan, UT) and plasma Cl − by the silver titration method using a Buchler-Cotlove digital chloridometer (Labconco, Kansas City, MO).

Techniques: Clinical Proteomics, Gene Expression, Saline, Injection

Journal: Journal of molecular endocrinology

Article Title: clc-2c is regulated by salinity, prolactin and extracellular osmolality in tilapia gill

doi: 10.1530/JME-17-0144

Figure Lengend Snippet: Changes in plasma osmolality (A) and branchial ncc2 and clc-2c gene expression (B) at 3 and 6 h after transfer of tilapia from fresh water to 30‰ brackish water. Mean ± SEM (n = 6). Gene expression is presented as a fold-change from time 0. For a given parameter, means not sharing the same letter are significantly different (one-way ANOVA, Tukey’s HSD test, P < 0.05).

Article Snippet: Plasma was separated by centrifugation for measurement of plasma osmolality using a vapor pressure osmometer (Wescor 5100C, Logan, UT) and plasma Cl − by the silver titration method using a Buchler-Cotlove digital chloridometer (Labconco, Kansas City, MO).

Techniques: Clinical Proteomics, Gene Expression

Journal: Journal of molecular endocrinology

Article Title: clc-2c is regulated by salinity, prolactin and extracellular osmolality in tilapia gill

doi: 10.1530/JME-17-0144

Figure Lengend Snippet: Effects of medium osmolality on clc-2c gene expression in gill filaments incubated in various osmolalities for 3 (A) and 6 h (B). Mean ± SEM (n = 8). Gene expression is presented as a fold-change from the 330 mOsm/kg group at 3 h. Means not sharing the same letter are significantly different (one-way ANOVA, Tukey’s HSD test, P < 0.05).

Article Snippet: Plasma was separated by centrifugation for measurement of plasma osmolality using a vapor pressure osmometer (Wescor 5100C, Logan, UT) and plasma Cl − by the silver titration method using a Buchler-Cotlove digital chloridometer (Labconco, Kansas City, MO).

Techniques: Gene Expression, Incubation